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Beyond CRISPR: A guide to the many other ways to edit a genome

106 pointsby oldbuzzardalmost 9 years ago

4 comments

xtacyalmost 9 years ago
This week&#x27;s Kurzgesagt episode covered CRISPR in an easy to understand fashion: <a href="https:&#x2F;&#x2F;www.youtube.com&#x2F;watch?v=jAhjPd4uNFY" rel="nofollow">https:&#x2F;&#x2F;www.youtube.com&#x2F;watch?v=jAhjPd4uNFY</a>.
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reasonattlmalmost 9 years ago
The big challenge at the moment is coverage; getting enough of the cells successfully edited by the gene therapy to make a meaningful difference. There are promising signs in mice, such as the muscular dystrophy study from earlier this year [1], but in general this is still an ongoing battle, without a standard approach.<p>The coverage issue is why you can&#x27;t go out and get any one of a hundred edits [2] and expect things to happen as a result. As soon as that is robustly solved, well, gene therapies will be out there in the medical tourism market, starting with telomerase, myostatin, and follistatin, I&#x27;d imagine. Most likely the second of those up front since its already fairly robustly proven in humans via antibody therapies. [3]<p>I would be extraordinarily surprised to find that there were no humans already tinkering with their myostatin balance via genes or antibodies, given the number of people with access to the technology, and the ease with which such a therapy could be assembled.<p>[1]: <a href="http:&#x2F;&#x2F;www.nature.com&#x2F;cr&#x2F;journal&#x2F;v26&#x2F;n5&#x2F;full&#x2F;cr201628a.html" rel="nofollow">http:&#x2F;&#x2F;www.nature.com&#x2F;cr&#x2F;journal&#x2F;v26&#x2F;n5&#x2F;full&#x2F;cr201628a.html</a><p>[2]: <a href="https:&#x2F;&#x2F;www.fightaging.org&#x2F;archives&#x2F;2016&#x2F;06&#x2F;a-short-list-of-potential-target-genes-for-near-future-gene-therapies-aimed-at-slowing-aging-or-compensating-for-age-related-damage-and-decline&#x2F;" rel="nofollow">https:&#x2F;&#x2F;www.fightaging.org&#x2F;archives&#x2F;2016&#x2F;06&#x2F;a-short-list-of-...</a><p>[3]: <a href="http:&#x2F;&#x2F;news.iupui.edu&#x2F;releases&#x2F;2015&#x2F;12&#x2F;myostatin-warden-muscle-growth.shtml" rel="nofollow">http:&#x2F;&#x2F;news.iupui.edu&#x2F;releases&#x2F;2015&#x2F;12&#x2F;myostatin-warden-musc...</a>
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dekhnalmost 9 years ago
It&#x27;s nice there are more gene editing methods but this just moves the problem back where it always belonged: delivery of the vector to the cells that matter, and knowing what edit to make are still extremely hard problems. The latter seems to be the ultimate challenge: our current understanding of genomics is still very much stuck in the &quot;the function of a gene is assumed to be the largest eigenvalue of its principle component analysis&quot;. In complex, redundant systems with feedback (like the genome and its associated cellular machinery) it&#x27;s basically impossible to fix an actual problem, with limited side effects, by just modifying a single gene.
incialmost 9 years ago
Ok, so while irrational, what makes this not mean that FOXDIE is about to become a reality?
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