I'm not sure about biggest, but on of the biggest sure.<p>Two problems.<p>1. DNA sequencing, now is two part process (I simplify a lot, sure) - at first stage, target DNA divided for small pieces, and those pieces sequenced to digital codes and saved to database.
Than in second part, computer cluster works few months, to connect those pieces to one chain.
And this is very expensive process even considering few gigabytes storage to store all these data for few months.<p>The problem is that currently used algorithms are very naive, even in some projects used Perl implementation, not C or some other fast language.
So exist opportunity, to create new special algorithm/software, or may be also some hardware support, so this will be at least 5 times faster, and if costs will drop under 100$ for one human DNA, this will lead to very new type of medicine - genetic checks of everything.<p>2. connected to 1st problem - find protein folding structures from DNA, and how they interact with other proteins, with drugs and with chemical molecules.
As I know, this now solved in similar way as 1st problem, but in partially 3d space - programs try to calculate some positions, in which parts of structures are attracted to each other and calculate power of attraction.
This way for example, calculated probability of drugs, which will attract to spike protein of Covid and neutralize it.
Also this way calculated effects of new drugs, like interaction with proteins in human organism, etc.<p>In ideal world, possible, that for example for cancer, 1st will create fast DNA of cancer cells of some human, and 2nd will create DNA code of artificial protein, which will be printed on DNA-printer and inhibit cancer, but will not affect normal cells, and all this will be done in just few weeks.