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Everything You Ever Wanted to Know about E. Coli (2008)

42 pointsby angrygoat9 months ago

3 comments

koeng9 months ago
I use E.coli on a pretty daily basis for cloning. It&#x27;s alright, and there is so much work that has gone into it as a chassis organism, but overall there are definitely better organisms out there if we just took the time to figure them out (Vibrio natriegens and Bacillus subtilis are two examples).<p>We absolutely do not have a clear idea of how E.coli works. Hell, we don&#x27;t even know how almost 1&#x2F;3 of the genes work on JCVI-Syn3a works, a minimal genome we synthetically created. Far fewer in E.coli.
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pazimzadeh9 months ago
The article touches on the versatility of E. coli, but doesn&#x27;t explicitly mention the extreme diversity within what we call E. coli.<p>Even just within the subset of E. coli which causes UTI&#x27;s, 25-40% of the genome varied between strains. (<a href="https:&#x2F;&#x2F;www.ncbi.nlm.nih.gov&#x2F;pmc&#x2F;articles&#x2F;PMC5653229&#x2F;" rel="nofollow">https:&#x2F;&#x2F;www.ncbi.nlm.nih.gov&#x2F;pmc&#x2F;articles&#x2F;PMC5653229&#x2F;</a>)<p>This diversity wasn&#x27;t really appreciated in 2008 when many E. coli genomes hadn&#x27;t been sequenced yet.
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mncharity9 months ago
<i>Many</i> numbers for E. coli: <a href="https:&#x2F;&#x2F;bionumbers.hms.harvard.edu&#x2F;search.aspx?trm=coli" rel="nofollow">https:&#x2F;&#x2F;bionumbers.hms.harvard.edu&#x2F;search.aspx?trm=coli</a>